The applied biosystems 7500 fast real time pcr offers an intuative and flexible solution for rapid detection and quantification of nucleic acids. Purchase an applied biosystems memory upgrade service. Data was collected on an applied biosystems 7500 fast realtime instrument 96well, 20 l reactions. Your 7500 fast or 7500 system can distinguish between samples containing 5,00010,000 dna template copies, with a statistical confidence level of 99. This applied biosystem prism 7000 sequence detection system is in excellent condition.
We have 4 applied biosystems 7500 manuals available for free pdf download. Applied biosystems stepone and steponeplus realtime pcr systems reagent guide ix preface examples of the user attention words appear below. The calibrate function is also available in the control console. Na na na na na na na na applied biosystems 7500 fast realtime pcr system contact information life technologies 850 lincoln centre drive. The abi 7500 fast dx real time pcr delivers the abi manual outlines how to this used lab equipment listing is for an abi 7500 fast realtime pcr system. Applied is here to provide you with more information, answer any questions you may have and deliver innovative solutions to enable your digital strategy. The abi prism 7000 sequence detection system qpcr rtpcr 7000 sds instrument is a secondgeneration sequence detection instrument capable of quantitative and qualitative detection with fluorescentbased pcr chemistries. Applied biosystems 4479030 service assembly new excitation spyder rc. Applied biosystems 7900ht fast realtime pcr system and sds. A service engineer installed fast upgrade kit is available for the 7500 system to upgrade it to the fast configuration. Applied biosystems 7300 real time pcr system youtube. The brand is focused on integrated systems for genetic analysis, which include computerized machines and the consumables used within them such as reagents in 2008, a merger between applied biosystems and invitrogen was finalized, creating life technologies. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support representative.
Performance was evaluated using synthetic twist sarscov2 rna control 2 diluted in 10 ng of jurkat total rna. Applied biosystems 75007500 fast realtime pcr systems. The applied biosystems 7500 is todays top choice in rt pcr. Other machines similar to applied biosystems 7500, fast realtime pcr. The plexor hy system is a novel realtime pcr system for quantitation of total human autosomal and human male y dna within a sample. Applied biosystems applied biosystems 7500 fast dx realtime pcr instrument, with tower computer 7500 fast dx rtpcr instrument with sds software is a realtime nucleic acid amplification and fivecolor fluorescence detection system available for in vitro diagnostic use. Abi applied biosystems 7500 realtime pcr system sonoran. Abi 7500 fast uses detection systems that employ realtime taqman polymerase chain reaction amplification of genes on a peltierbased thermocycling apparatus, or customerdesigned sybrbased assays. Applied biosystems 7500 applied biosystems 7500 fast applied biosystems viia 7 stratagene mx3000p. View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. The applied biosystems 7500 fast realtime pcr system delivers high speed cycling capabilities with a new fast ramping peltierbased thermal cycling block. The applied biosystems 7500 and 7500 fast realtime pcr systems are integrated, versatile platforms for the detection and quantification of nucleic acid sequences.
Once installed, an applied biosystems field service engineer will ensure your 7500 fast or 7500 system is performing instrument verification plate, we will verify that. The 7500 realtime pcr system is a sophisticated platform for users who require extended capabilities and maximum versatility. Applied biosystems 7500 fast and 7500 realtime pcr systems. Sales inquiry reach out to our sales team directly for immediate assistance with all salesrelated inquiries. Thermo fisher scientific 7500 pcr system community. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well format. From the instrument menu at the top of the screen, select instrument maintenance manager. Reactions were run in duplicate using 10fold dilutions of human leukocyte rna 100 ng to 0. Applied biosystems 7500 fast real time pcr w 96well block. Applied biosystems 7500 7500 fast realtime pcr software 7500 software. The applied biosystems 7500 fast dx realtime pcr instrument, with sds v1. The technical manual listed below, complete protocol, describes the plexor hy system and provides instructions for use with the applied biosystems 7500 and 7500 fast realtime pcr systems, including reaction setup, realtime pcr instrument.
Intended for laboratory staff and principal investigators who perform experiments using the 7500 7500 fast system. An optional upgrade to the 7500 fast system is available. The technical manual listed below, complete protocol, describes the plexor hy system and provides instructions for use with the applied biosystems 7500 and 7500 fast realtime pcr systems, including reaction setup, realtime pcr instrument programming and data analysis using the plexor analysis software. We also offer the 7500 fast dx instrument for your diagnostic needs. The applied biosystems 7500 fast dx realtime pcr instrument, with sequence detection system sds software, is a 96well, 5color realtime pcr instrument available for in vitro diagnostic use. Applied biosystems 7500 fast, 7500 and 7300 realtime pcr systems. Applied biosystems 75007500 fast realtime pcr system getting.
Intended for laboratory staff responsible for the maintenance of the 75007500 fast system. Applied biosystems 7500 fast installation and maintenance manual 174 pages. This third generation platform features an innovative optical system that enhances sensitivity. Applied biosystems 7500 anleitung pdfherunterladen. The guides listed below are shipped with the applied biosystems 75007500 fast.
Results in 30 minutes fully optimized for fast cycling, the 7500 fast system delivers highquality results in as little as 30 minutes. This instrument is capable of quantitative detection using real. Applied biosystems 7300 7500 7500 fast realtime pcr system installation and maintenance getting started guide pn 4347828 taqman fast universal pcr master mix 2 protocol pn 4351891. Portable document format pdf versions of the following related documents are available on a cd which is shipped with the applied biosystems 7500 fast dx. Applied biosystems 7500 fast and 7500 realtime pcr system. Applied biosystems is a global leader in providing innovative instruments and reagents to accelerate academic and clinical research. Description the applied biosystems 7500 fast realtime pcr system offers maximum performance in the minimum time. The applied customer experience team is committed to providing services, support and education and training as your partner in digital transformation. Abi 7500 and 7500 fast realtime pcr system applied biosystems 7500 software, version 2. Installation and maintenance manual, getting started, getting started manual. The applied biosystems 7500 realtime pcr system is however slight improvements have made this program more versatile and user the abi 7500 fast system. Licensing at applied biosystems, 850 lincoln centre drive, foster city, california 94404, or the licensing department, roche molecular systems, inc. Leverage best practices to seamlessly transition to new technology.
Realtime pcr combines thermal cycling, fluorescence detection, and applicationspecific software to measure the cyclebycycle accumulation of pcr products in a singletube homogenous reaction. Applied biosystems 7500 fast real time pcr is available from the lab world group. Documents document view page applied biosystems 2720. If you would like an applied biosystems service engineer to upgrade the memory of your computer, you can purchase a memory upgrade service from the applied biosystems customer care center. Applied biosystems abi 7500 real time pcr video youtube. Their easy to follow procedures, eight 8 sample allotments in a 96test kit, and robust stability are compatible with an existing workflow. Applied systems contact information applied systems.
Applied biosystemsapplied biosystems 7500 fast dx real. Applied biosystems 7500 fast realtime pcr system, laptop. Choose from our standard 7500 pcr systems or the 7500 fast dx instrument for your diagnostic needs. Applied biosystems 7300 real time pcr system available for sale at the lab world group. Community forums for applied biosystems 7500 relating to 7500 realtime pcr system service manual on labwrench. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning and genotyping. To verify your client connection, you need a valid user id. The applied biosystems 7500 fast realtime pcr system is a realtime pcr system which enables highspeed thermal cycling with run times for quantitative realtime pcr applications of fewer than 40 minutes. Applied biosystems 75007500 fast realtime pcr systems user. Applied biosystems 7500 and 7500 fast software version 2. Abi prism, applied biosystems, geneamp, primer express, sybr, and vic are registered. To facilitate many of these applications, applied biosystems provides preformulated, readytouse, qualitytested, taqman assays for use with the 7500 system. Now you can reduce your assay optimization efforts.
Used applied biosystems abi, 7500, pcr for sale 261942355. Our global team of more than 80,000 colleagues delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industryleading brands, including thermo scientific, applied biosystems, invitrogen, fisher scientific, unity. The applied biosystems 7500 realtime pcr system complete validation solutions applied biosystems offers iqoq, a validation support solution. View online or download applied biosystems 7500 fast installation and maintenance manual, getting started. Thats why they are preferred by federal and local government agencies for human. Applied biosystems 7500 realtime pcr system, laptop. Applied biosystems 7500 fast installation and maintenance manual 174 pages realtime pcr system. Under these conditions, the luna kit has an lod of 5 copiesreaction for both targets while the lod using taqpath is 10 copies. Applied biosystems 7500 and 7500 fast realtime pcr systems are integrated, versatile platforms for the detection and quantification of nucleic acid sequences. Applied biosystems 75007500 fast realtime pcr system maintenance guide explains how to maintain the 75007500 fast system. Biosystems 7300 7500 real time pcr system absolute quantitation getting started guide, or the applied biosystems 7300 7500 real time pcr system installation and maintenance getting started guide for limited label license or disclaimer information. The abi 7500 is a real time pcr that delivers accurate results in less than 30 minutes. Our systems offer dye flexibility to generate reliable data.
Applied biosystems 7500 fast, 7500 and 7300 realtime pcr. Pronex dna qc assay calibration kit, 7500 technical manual. The system is an open platform and can be used to analyze samples using custom made or cotsbased pcr assays. Make offer refurbished applied biosystems abi 7500 fast realtime pcr system, year 2015 applied biosystems ab 4700 module 16 channel ospi master pn.
This technical manual describes the pronex dna qc assay dye calibration kit, 7500 and provides instructions for use with the applied biosystems abi 7500 and 7500 fast realtime pcr system applied biosystems 7500 software, version 2. Applied biosystems 7500 fast dx realtime pcr instrument. Lyra assays have been validated on the applied biosystems 7500 fast dx realtime pcr instrument, cepheid smartcycler ii and life technologies quantstudiotm dx realtime pcr instrument. Applied biosystems abi applied biosystems pcr applied biosystems 2720 thermal cycler tags. Applied biosystems 7500 realtime pcr system thermo fisher. Applied biosystems 75007500 fast thermo fisher scientific. The applied biosystems 7500 fast realtime pcr system offers maximum performance in the minimum time. You can also access pdf versions of all documents in the applied biosystems. Applied biosystems is one of the well known brands manufactured by thermo fisher scientific. Service and support available throughout the entire process same day turnaround time applied biosystems 7500 fast dx realtime pcr system or 7500 fast realtime pcr system 7500 realtime pcr system quantstudio5 realtime pcr system 115 min applied biosystems covid19 interpretive software magmax viralpathogen nucleic acid. Realtime pcr abi prism 7500 fast psc 535, nsc 338a.
When designing realtime pcr primers, keep in mind that the amplicon length should be approximately 50150 bp, since longer. Plexor hy system for the applied biosystems 7500 and 7500 fast realtime pcr systems technical manual. The 7500 realtime pcr systems consistently deliver highperformance multicolor realtime pcr. Applied biosystems 7500 fast real time pcr youtube. Applied biosystems 2720 thermal cycler user manual. Oqipv service for applied biosystems realtime pcr systems.
Real time pcr model applied biosystems 7500 now on sale at the lab world group. The applied biosystems 7500 fast realtime pcr system offers maximum performance in the minimum time for labs running a variety of applications, including high resolution melting hrm analysis. Applied biosystems is one of the various brands under the life technologies brand of thermo fisher scientific corporation. Applied biosystems stepone steponeplus user manual 5book paperback pack rev c. Relative quantitation using comparative ct on 7300 7500 7500 fast sds v1. Refurbished applied biosystems abi 7500 fast realtime pcr system, year 2015. To order documents, download pdf files, or for help with a. Applied biosystems 7500 the applied biosystems 7500 realtime pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. An engineer can perform the upgrade service during a planned maintenance visit at an additional cost. Applied biosystems 7500 fast dx realtime pcr instrument user guide for the security, auditing, and esignature module in sds software v1. Applied biosystems 7500 realtime pcr systems offer you high performance, multicolor realtime pcr wherever your research takes you.
The applied biosystems 7500 realtime pcr system is a powerful platform 7500 fast and 7500 realtime pcr systems 7500 realtime pcr system service manual. Each symbol may appear by itself or in combination with text that explains the relevant hazard see safety labels on instruments on page xii. Laboratory manual, third edition, cold spring harbor laboratory press, new york 2000. The 7500 fast realtime pcr system enables standard 96well format high speed thermal cycling, significantly reducing your run time for quantitative realtime pcr applications, deliv ering results in about 35 minutes the 7500 realtime pcr system is a versatile, leadingedge platform providing enhanced performance capabilities and an upgrade path to highspeed thermal cycling fivecolor detection system provides the. Applied biosystems 7500 realtime pcr system applied biosystems 7300 realtime pcr system primer express software lets you independently design oligonucleotides oligos for. Find online auctions and classified ads for applied biosystems products on the labx marketplace. Thermo fisher scientific 7500 pcr system community, manuals. Browse ads and contact the seller directly or request a quote for more details. Applied biosystems 7500 manuals manuals and user guides for applied biosystems 7500. A realfast and real versatile approach to realtime pcr. Applied biosystems stepone and steponeplus realtime pcr systems reagent guide vii preface how to use this guide about the system documentation the guides listed below are shipped with the applied biosystems stepone and steponeplus realtime pcr systems stepone and steponeplus systems. Customers may developdesign their own assays or take advantage of applied biosystems life technologies predesigned, fullyoptimized assays. This real time pcr system combines pre boxed or custom assays, and advanced thermal cycling systems with responsive software for a vast array of applications.
Applied biosystems 75007500 fast realtime pcr software 7500 software. Safety symbols the following table describes the safety symbols that may be displayed on applied biosystems instruments. The applied biosystems 7500 realtime pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. Installation and maintenance guide pn 4347828e thermo fisher. Plexor hy system for the applied biosystems 7500 and 7500.
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